A simple, rapid, accurate, precise and economical reverse phase high performance liquid chromatographic method is developed for simultaneous separation and quantification of two anti-hypertensive drugs, viz., chlorthalidone and olmesartan medoxomil. The separation of both the drugs was achieved on Inertsil ODS 3V C18 column (250 x 4.6 mm id, 5 µm particle size) column using a mobile phase of sodium dihydrogen phosphate buffer solution (at pH 3): acetonitrile (48:52 v/v). The flow rate was 1 ml/min and detection was done at 270 nm. The retention time for chlorthalidone was 3.65 min and olmesartan medoxomil was 6.23 min. Chlorthalidone and olmesartan medoxomil showed a linear response in the concentration range of 6.25-37.50 µg/ml and 10-60 µg/ml respectively. The correlation co-efficients for Chlorthalidone and olmesartan medoxomil were 0.9999 and 0.9998 respectively. The percentage recoveries obtained for chlorthalidone and olmesartan medoxomil ranges from 99.5% to 100.1% and 99.1% to 100.4% respectively. The results of analysis have been validated as per ICH guidelines. Validation results indicated that method shows satisfactory linearity, accuracy, precision and ruggedness. The extremely low flow rate, simple mobile phase composition makes this method cost effective, rapid and non-tedious and can also be successfully employed for simultaneous estimation of both drugs in commercial products.
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